File(s) not publicly available
Induction of human regulatory T cells with bacterial superantigens
chapter
posted on 2023-06-08, 23:48 authored by Stefano Caserta, Amanda L Taylor, Nadia Terrazzini, Martin LlewelynMartin LlewelynRegulatory T cells (Tregs) that suppress the activation of immune effector cells limit immunopathology and are fast emerging as therapeutic targets for autoimmune and cancer disease. Tools enabling Treg in vitro-induction, expansion, and characterization and manipulation will help future clinical developments. In this chapter, we describe in detail how to use bacterial superantigens to induce human Tregs efficiently from peripheral blood mononuclear cells. How to assess human Treg phenotype and suppressive capacity are also described. Technical details, variations, and alternative experimental conditions are provided.
History
Publication status
- Published
Journal
Methods in molecular biology (Clifton, N.J.)ISSN
1940-6029Publisher
Springer New YorkExternal DOI
Volume
1396Page range
181-206Book title
SuperantigensPlace of publication
New YorkISBN
9781493933426Series
Methods in Molecular BiologyDepartment affiliated with
- Clinical and Experimental Medicine Publications
Full text available
- No
Peer reviewed?
- Yes