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Intracellular microbe whole-genome expression profiling: methodological considerations and biological inferences
Understanding the mechanisms by which microbes survive in their host intracellular environments is an essential precursor to developing novel intervention strategies that might exploit the interplay between host and microbe. Transcriptional profiling allows us a glimpse of these molecular interactions and promises to reveal much of this dialogue in both pathogenic and symbiotic settings. Such studies will underpin the development of antivirulence strategies for new therapeutics. This chapter aims to describe the challenges of studying microbial RNA expression from intracellular and in vivo scenarios, and to detail a fraction of the insights acquired from this rapidly expanding field, driven by the recent availability of microbe whole-genome sequence information.
History
Publication status
- Published
File Version
- Accepted version
Publisher
Wiley-VCH PublishersExternal DOI
Pages
145.0Book title
Intracellular Niches of Microbes– A Pathogen's Guide through the Host CellISBN
9783527322077Department affiliated with
- Global Health and Infection Publications
Full text available
- No
Peer reviewed?
- Yes