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Whole genome analysis using microarrays
chapter
posted on 2023-06-07, 16:23 authored by Simon WaddellSimon Waddell, Jason Hinds, Philip D ButcherThe development of microarray technology has allowed the genomes of mycobacteria to be directly compared to identify DNA regions that differ between strains due to deletion, insertion, or sequence divergence. The use of microarrays in comparative genomics has proved to be a valuable tool for comparing both mycobacterial species and strains. We describe here the methodology for comparing two mycobacterial DNA samples by microarray hybridization, from labeling and slide preparation, to DNA microarray analysis options. Further developments in microarray design and methodology promise to ensure that microarrays remain an important resource for comparative genomic studies in the future.
History
Publication status
- Published
File Version
- Accepted version
Journal
Methods in molecular biology (Clifton, N.J.)ISSN
1940-6029Publisher
SpringlerExternal DOI
Volume
465Page range
1-12Book title
Mycobacteria Protocols : Second EditionPlace of publication
Totowa, NJISBN
978-1-58829-889-8Series
Methods in Molecular BiologyDepartment affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes