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A wide field fluorescence lifetime imaging system using a light sheet microscope
conference contributionposted on 2023-06-09, 01:06 authored by Phil BirchPhil Birch, Lamar Moore, Xiaofei Li, Roger Phillips, Rupert YoungRupert Young, Chris ChatwinChris Chatwin
Fluorescence lifetime imaging microscopy (FLIM) has allowed scientists to discern information about the chemical properties of biological processes and has become a vital tool in the life sciences and medical research communities. Measuring the spatial lifetime distribution of the fluorophores as well as the intensity distribution enables users to discern vital information about the chemical environment. It however, remains challenging and often involves slow scanning. We present a new microscope system based on light sheet illumination that uses a micro channel plate (MCP) device called a Capacitive Division Imaging Readout (CDIR) which has been developed by Photek Ltd. The device uses an array of capacitors to move the charge site from the MCP to four pre-amplifiers and time-over-threshold discriminators. This camera has the ability to image photons as well as measure the arrival time, enabling high speed FLIM imaging of biological samples.
- Accepted version
JournalProceedings of SPIE 2016 on Biophotonics: Photonic Solutions for Better Health Care; Brussels, Belgium; 4th-7th April 2016
PublisherSociety of Photo Optical Instrumentation Engineers
Department affiliated with
- Engineering and Design Publications
Research groups affiliated with
- Industrial Informatics and Signal Processing Research Group Publications
NotesCopyright 2016 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
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