University of Sussex
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Data from the behavioural experiments described in the paper: A CREB2-targeting microRNA is required for long-term memory after single-trial learning.

posted on 2022-10-07, 15:04 authored by Sergei Korneev, Dimitris V Vavoulis, Souvik Naskar, Varvara E Dyakonova, Ildiko KemenesIldiko Kemenes, George KemenesGeorge Kemenes
Data for paper appearing in Scientific Reports.

Excel workbooks with unconditioned and conditioned feeding response data obtained in behavioural pharmacological experiments exploring the role of microRNAs in general and miR-137 in particular in long-term memory after single-trial classical conditioning.

Abstract from research paper
Although single-trial induced long-term memories (LTM) have been of major interest in neuroscience, how LTM can form after a single episode of learning remains largely unknown. We hypothesized that the removal of molecular inhibitory constraints by microRNAs (miRNAs) plays an important role in this process. To test this hypothesis, first we constructed small non-coding RNA (sncRNA) cDNA libraries from the CNS of Lymnaea stagnalis subjected to a single conditioning trial. Then, by next generation sequencing of these libraries, we identified a specific pool of miRNAs regulated by training. Of these miRNAs, we focussed on Lym-miR-137 whose seed region shows perfect complementarity to a target sequence in the 3’ UTR of the mRNA for CREB2, a well-known memory repressor. We found that Lym-miR-137 was transiently up-regulated 1 h after single-trial conditioning, preceding a down-regulation of Lym-CREB2 mRNA. Furthermore, we discovered that Lym-miR-137 is co-expressed with Lym-CREB2 mRNA in an identified neuron with an established role in LTM. Finally, using an in vivo loss-of-function approach we demonstrated that Lym-miR-137 is required for single-trial induced LTM.


This work was funded by grants from the Medical Research Council to G.K. (MRC/G0400551), and the BBSRC to G.K and I.K. (BB/H009906/1) and to I.K. and G.K.(BB/P00766X/1), respectively.

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