Excel workbooks with unconditioned and conditioned feeding response data obtained in behavioural pharmacological experiments exploring the role of microRNAs in general and miR-137 in particular in long-term memory after single-trial classical conditioning.
Abstract from research paper Although single-trial induced long-term memories (LTM) have been of
major interest in neuroscience, how LTM can form after a single episode
of learning remains largely unknown. We hypothesized that the removal of
molecular inhibitory constraints by microRNAs (miRNAs) plays an
important role in this process. To test this hypothesis, first we
constructed small non-coding RNA (sncRNA) cDNA libraries from the CNS of
Lymnaea stagnalis subjected to a single conditioning trial.
Then, by next generation sequencing of these libraries, we identified a
specific pool of miRNAs regulated by training. Of these miRNAs, we
focussed on Lym-miR-137 whose seed region shows perfect complementarity
to a target sequence in the 3’ UTR of the mRNA for CREB2, a well-known
memory repressor. We found that Lym-miR-137 was transiently up-regulated
1 h after single-trial conditioning, preceding a down-regulation of Lym-CREB2 mRNA. Furthermore, we discovered that Lym-miR-137 is co-expressed with Lym-CREB2
mRNA in an identified neuron with an established role in LTM. Finally,
using an in vivo loss-of-function approach we demonstrated that
Lym-miR-137 is required for single-trial induced LTM.
Funding
This work was funded by grants from the Medical Research Council to G.K. (MRC/G0400551), and the BBSRC to G.K and I.K. (BB/H009906/1) and to I.K. and G.K.(BB/P00766X/1), respectively.
Analysis of the cellular and molecular mechanisms of memory function and dysfunction in a defined neuronal network