Dataset for paper:Identification of a Novel Retrieval-dependent Memory Process in the Crab Neohelice granulata

Data for paper published in Neuroscience (Vol. 448), 10/11/2020

Each file is named according to the corresponding figure panel and contains pre-processed information of each animal's tracking. Data represents position or each animal in x and y coordinates, respectively.

For each figure panel, you will find two Excel flaps: one containing data from the pre-training trial and another with data from the test trial.

Each row corresponds to a single frame of each analysed video and each video has a duration of 9 seconds with an acquisition rate of 10 frames per second (total of 90 frames). Yellow highlighting indicates those frames in which the US occurs.

In the columns the data is organised according to the group to which they belong (ABCD in experiments of 4 groups or ABCDEF in experiments of 6 groups) and

according to the number of the animal (from 1 to 40). Missing data correspond to animals that died during the experiment, typically due to the injection procedure.

Abstract

Fully consolidated associative memories may be altered by alternative retrieval dependent memory processes. While a brief exposure to the conditioned stimulus (CS) can trigger reconsolidation of the original memory, a prolonged CS exposure will trigger memory extinction. The conditioned response is maintained after reconsolidation, but is inhibited after extinction, presumably by the formation of a new inhibitory memory trace. In rats and humans, it has been shown that CS exposure of intermediate duration leave the memory in an insensitive or limbo state. Limbo is characterised by the absence of reconsolidation or extinction. Here we investigated the evolutionary conserved nature of limbo using a contextual Pavlovian conditioning (CPC) memory paradigm in the crab Neohelice granulata. In animals with fully consolidated CPC memory, systemic administration of the protein synthesis inhibitor cycloheximide after 1 CS presentation disrupted the memory, presumably by interfering with memory reconsolidation. The same intervention given after 320 CSs prevented CPC memory extinction. Cycloheximide had no behavioural effect when administered after 80 CS presentations, a protocol that failed to extinguish CPC memory. Also, we observed that a stronger CPC memory engaged reconsolidation after 80 CS instead of limbo, indicating that memory strength affects the parametrical conditions to engage either reconsolidation or limbo. Altogether, these results indicate that limbo is an evolutionary conserved memory process segregating reconsolidation from extinction in the number of CSs space. Limbo appears as an intrinsic component of retrieval dependent memory processing, with a key function in the transition from memory maintenance to inhibition.