1/1
7 files

Research data for paper ‘Interneuronal mechanisms for learning-induced switch in a sensory response that anticipates changes in behavioural outcomes’.

dataset
posted on 09.02.2021, 13:55 by Zsolt Pirger, Zita Laszlo, Souvik Naskar, Michael CrossleyMichael Crossley, Michael O'Shea, Paul R Benjamin, George KemenesGeorge Kemenes, Ildiko KemenesIldiko Kemenes
Behavioural and electrophysiological data used in the figures for paper published in Current Biology 10 February 2021.

Datasets:
Fig. 1. Behavioural data obtained by testing animals before and after aversive classical conditioning.
Fig. 2D. Data obtained by electrophysiologically testing the sucrose response of the interneurone PlB in semi-intact preparations.
Fig. 2E. Data obtained by measuring the baseline membrane potential and firing frequency of the interneurone PlB in semi-intact preparations.
Fig. 3D. Data obtained by electrophysiologically testing the sucrose response of the feeding motoneuron B3 during the application of sucrose, before and after the photoinactivation of the interneurone PlB.
Fig. 4C. Data obtained by electrophysiologically testing the response of the interneurone PlB to the electrical stimulation of the interneurone PeD12.
Fig. S1. 1. Behavioural data obtained by testing animals before and after control protocols for aversive classical conditioning.


Abstract

Sensory cues in the natural environment predict reward or punishment, important for survival. For example, the ability to detect attractive tastes indicating palatable food is essential for foraging whilst the recognition of inedible substrates prevents harm. Whilst some of these sensory responses are innate, they can undergo fundamental changes due to prior experience associated with the stimulus. However, the mechanisms underlying such behavioural switching of an innate sensory response at the neuron and network levels require further investigation. We used the model learning system of Lymnaea stagnalis [1-3] to address the question of how an anticipated aversive outcome reverses the behavioural response to a previously effective feeding stimulus, sucrose. Key to the switching mechanism is an extrinsic inhibitory interneuron of the feeding network, PlB (pleural buccal [4, 5]), which is inhibited by sucrose to allow a feeding response. After multi-trial aversive associative conditioning, pairing sucrose with strong tactile stimuli to the head, PlB’s firing rate increases in response to sucrose application to the lips and the feeding response is suppressed; this learned response is reversed by the photoinactivation of a single PlB. A learning-induced persistent change in the cellular properties of PlB that results in an increase rather than a decrease in its firing rate in response to sucrose provides a neurophysiological mechanism for this behavioural switch. A key interneuron, PeD12 (Pedal-Dorsal 12), of the defensive withdrawal network [5, 6] does not mediate the conditioned suppression of feeding but its facilitated output contributes to the sensitization of the withdrawal response.




Funding

Decision Making and Learning in Neuronal Networks

Biotechnology and Biological Sciences Research Council

Find out more...

Updating of memories during memory consolidation

Biotechnology and Biological Sciences Research Council

Find out more...

National Brain Research Programme (Hungary) grant 2017-1.2.1-NKP-2017-00002

History