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Research data for paper Molecular and functional characterization of an evolutionarily conserved CREB-binding protein in the Lymnaea CNS

dataset
posted on 10.10.2022, 18:47 authored by Dai Hatakeyama, Hiroshi Sunada, Yuki Totani, Takayuki Watanabe, Ildikó Felletár, Adam FitchettAdam Fitchett, Murat Eravci, Aikaterini Anagnostopoulou, Ryosuke Miki, Ayano Okada, Naoya Abe, Takashi Kuzuhara, Ildiko KemenesIldiko Kemenes, Etsuro Ito, George KemenesGeorge Kemenes

Research data for paper Molecular and functional characterization of an evolutionarily conserved CREB-binding protein in the Lymnaea CNS (FASEB Journal October 2022).


This dataset consists of two Excel spreadsheets containing molecular biological and electrophysiological data, respectively.


The data used for Figure 2 was obtained by measuring the expression levels of the CBP mRNA in different tissues of Lymnaea by using qRT-PCR. The data used for the graph and associated statistical analysis show that the expression level is highest in the central nervous system (CNS).


The data used for Figure 6F was obtained by intracellular microelectrophysiology to measure the amplitude of the monosynaptic EPSP evoked in the motoneuron B1 by the electrical stimulation of the Cerebral Giant Cell (CGC) under different experimental conditions. The data used for the graph and associated statistical analysis show that injection of cAMP into the CGC cell body increased EPSP amplitude in the B1 motoneuron, but pre-treatment with either of the three HAT inhibitors prevented the cAMP-induced increase of EPSP. It also shows that 5'AMP that was used as a control for cAMP did not increase the amplitude of the B1 EPSP.

Funding

Decision Making and Learning in Neuronal Networks

Biotechnology and Biological Sciences Research Council

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Updating of memories during memory consolidation

Biotechnology and Biological Sciences Research Council

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Analysis of the cellular and molecular mechanisms of memory function and dysfunction in a defined neuronal network

Medical Research Council

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History