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A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus
journal contribution
posted on 2023-06-07, 13:33 authored by Isabelle Dionne, Ravi K Nookala, Stephen P Jackson, Aidan DohertyAidan Doherty, Stephen D BellThe sliding clamp, PCNA, of the archaeon Sulfolobus solfataricus P2 is a heterotrimer of three distinct subunits (PCNA1, 2, and 3) that assembles in a defined manner. The PCNA heterotrimer, but not individual subunits, stimulates the activities of the DNA polymerase, DNA ligase I, and the flap endonuclease (FEN1) of S. solfataricus. Distinct PCNA subunits contact DNA polymerase, DNA ligase, or FEN1, imposing a defined architecture at the lagging strand fork and suggesting the existence of a preformed scanning complex at the fork. This provides a mechanism to tightly couple DNA synthesis and Okazaki fragment maturation. Additionally, unique subunit-specific interactions between components of the clamp loader, RFC, suggest a model for clamp loading of PCNA.
History
Publication status
- Published
Journal
Molecular CellISSN
1097-2765Publisher
ElsevierExternal DOI
Issue
1Volume
11Page range
275-82Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2006-11-27Usage metrics
Categories
No categories selectedKeywords
Amino Acid Sequence Archaeal Proteins/chemistry/genetics/ metabolism DNAArchaeal/genetics/metabolism DNA-Directed DNA Polymerase/metabolism ModelsMolecular Molecular Sequence Data Proliferating Cell Nuclear Antigen/chemistry/genetics/ metabolism Protein Conformation Protein Subunits/metabolism Recombinant Fusion Proteins/metabolism Research SupportNon-U.S. Gov't Sequence Alignment Sulfolobus/ metabolism Two-Hybrid System Techniques