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A modified family-B archaeal DNA polymerase with reverse transcriptase activity
journal contribution
posted on 2023-06-08, 05:01 authored by Stanislaw Jozwiakowski, Bernard A ConnollyA hybrid capable of copying stretches of RNA template ~100 bases long is the first example of a thermostable B family polymerase/reverse transcriptase. The high-fidelity archaeal polymerase contains a fingers domain derived from the more error prone polymerase ?. For maximum RT activity, fusion with the processivity factor Sso7d is required and the proof-reading exonuclease must be disabled.
History
Publication status
- Published
Journal
ChemBioChemISSN
1439-4227Publisher
WileyExternal DOI
Issue
1Volume
12Page range
35-7Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- No
Peer reviewed?
- Yes