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Archaeal replicative primases can perform translesion DNA synthesis
journal contribution
posted on 2023-06-08, 20:17 authored by Stanislaw K Jozwiakowski, Farimah Borazjani Gholami, Aidan DohertyAidan DohertyDNA replicases stall at lesions during replication, potentially leading to genome instability. However, cells use specialized lesion bypass polymerases to restart stalled replisomes. Although most organisms possess these damage tolerance polymerases, capable of traversing blocking DNA lesions, many appear to lack these enzymes. We have discovered that replicative primases from archaea, previously considered to be solely involved in priming replication, are also capable of performing translesion DNA synthesis. This discovery has major implications for our understanding of additional roles of DNA primases during replication and the subsequent evolution of related lesion bypass pathways in eukaryotic organisms.
Funding
Molecular basis for repairing DNA double-strand breaks by non homologous end-joining; G0887; BBSRC-BIOTECHNOLOGY & BIOLOGICAL SCIENCES RESEARCH COUNCIL; BB/J018643/1
The role of a novel family of eukaryotic DNA polymerases in mitochondrial DNA replication; G0207; BBSRC-BIOTECHNOLOGY & BIOLOGICAL SCIENCES RESEARCH COUNCIL; BB/H019723/1
MRC; G080130
History
Publication status
- Published
Journal
Proceedings of the National Academy of SciencesISSN
0027-8424Publisher
National Academy of SciencesExternal DOI
Issue
7Volume
112Article number
E633-E638Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2015-03-11Usage metrics
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