University of Sussex
Browse

Are SMC complexes loop extruding factors? Linking theory with fact

Download (1018.83 kB)
Version 2 2023-06-12, 08:58
Version 1 2023-06-09, 16:36
journal contribution
posted on 2023-06-12, 08:58 authored by Jon BaxterJon Baxter, Antony OliverAntony Oliver, Stephanie A Schalbetter
The extreme length of chromosomal DNA requires organizing mechanisms to both promote functional genetic interactions and ensure faithful chromosome segregation when cells divide. Microscopy and genome-wide contact frequency analyses indicate that intra-chromosomal looping of DNA is a primary pathway of chromosomal organization during all stages of the cell cycle. DNA loop extrusion has emerged as a unifying model for how chromosome loops are formed in cis in different genomic contexts and cell cycle stages. The highly conserved family of SMC complexes have been found to be required for DNA cis-looping and have been suggested to be the enzymatic core of loop extruding machines. Here, the current body of evidence available for the in vivo and in vitro action of SMC complexes is discussed and compared to the predictions made by the loop extrusion model. How SMC complexes may differentially act on chromatin to generate DNA loops and how they could work to generate the dynamic and functionally appropriate organization of DNA in cells is explored.

Funding

Investigating the interplay between SMC complexes and Topoisomerase II; G2520; BBSRC; BB/S001425/1

How do Smc5/6 interactions with DNA coordinate replication and recombination?; G2119; MRC-MEDICAL RESEARCH COUNCIL; MR/P018955/1

History

Publication status

  • Published

File Version

  • Published version

Journal

BioEssays

ISSN

1521-1878

Publisher

Wiley

Issue

1

Volume

41

Page range

1800182 1-10

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Research groups affiliated with

  • Genome Damage and Stability Centre Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2019-01-22

First Open Access (FOA) Date

2019-01-22

First Compliant Deposit (FCD) Date

2019-01-21

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC