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Biotin-, fluorescein- and ‘clickable’ conjugates of phospha-oseltamivir as probes for the influenza virus which utilize selective binding to the neuraminidase
journal contribution
posted on 2023-06-07, 20:58 authored by Mathew Stanley, Max Birge, Stephen R Martin, Benoit Carbain, Hansjorg StreicherThe synthesis of conjugates of phospha-oseltamivir to the well established reporter groups fluorescein and biotin and an approach to multimeric inhibitors is described. We report powerful inhibition of the influenza neuraminidase by these probes and quantify fluorescence quenching during binding of the fluorescein conjugate through titration with the neuraminidase. Thus, we show that they could be useful tools to efficiently inhibit, detect and quantify the virus and the neuraminidase in biological systems.
History
Publication status
- Published
Journal
Organic and Biomolecular ChemistryISSN
1477-0520Publisher
Royal Society of ChemistryVolume
9Page range
5625-5629Department affiliated with
- Chemistry Publications
Full text available
- No
Peer reviewed?
- Yes