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Broader implications of SILAC-based proteomics for dissecting signaling dynamics in cancer
journal contribution
posted on 2023-06-08, 21:28 authored by Hua Zhang, Yichen Xu, Panos Papanastasopoulos, Justin Stebbing, Georgios GiamasGeorgios GiamasLarge-scale transcriptome and epigenome analyses have been widely utilized to discover gene alterations implicated in cancer development at the genetic level. However, mapping of signaling dynamics at the protein level is likely to be more insightful and needed to complement massive genomic data. Stable isotope labeling with amino acids in cell culture (SILAC)-based proteomic analysis represents one of the most promising comparative quantitative methods that has been extensively employed in proteomic research. This technology allows for global, robust and confident identification and quantification of signal perturbations important for the progress of human diseases, particularly malignancies. The present review summarizes the latest applications of in vitro and in vivo SILAC-based proteomics in identifying global proteome/phosphoproteome and genome-wide protein-protein interactions that contribute to oncogenesis, highlighting the recent advances in dissecting signaling dynamics in cancer.
History
Publication status
- Published
Journal
Expert Review of ProteomicsISSN
1478-9450Publisher
Expert ReviewsExternal DOI
Issue
6Volume
11Page range
713-731Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes