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Cdk activity couples epigenetic centromere inheritance to cell cycle progression
journal contribution
posted on 2023-06-08, 12:36 authored by Marina C C Silva, Dani L Bodor, Stellfox E Madison, Nuno M C Martins, Helfrid HocheggerHelfrid Hochegger, Daniel R Foltz, Lars E T JansenCentromeres form the site of chromosome attachment to microtubules during mitosis. Identity of these loci is maintained epigenetically by nucleosomes containing the histone H3 variant CENP-A. Propagation of CENP-A chromatin is uncoupled from DNA replication initiating only during mitotic exit. We now demonstrate that inhibition of Cdk1 and Cdk2 activities is sufficient to trigger CENP-A assembly throughout the cell cycle in a manner dependent on the canonical CENP-A assembly machinery. We further show that the key CENP-A assembly factor Mis18BP1(HsKNL2) is phosphorylated in a cell cycle-dependent manner that controls its centromere localization during mitotic exit. These results strongly support a model in which the CENP-A assembly machinery is poised for activation throughout the cell cycle but kept in an inactive noncentromeric state by Cdk activity during S, G2, and M phases. Alleviation of this inhibition in G1 phase ensures tight coupling between DNA replication, cell division, and subsequent centromere maturation
History
Publication status
- Published
Journal
Developmental CellISSN
1534-5807Publisher
ElsevierExternal DOI
Issue
1Volume
22Page range
52-63Department affiliated with
- Evolution, Behaviour and Environment Publications
Full text available
- No
Peer reviewed?
- Yes