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DNA polymerase ? is an A-T mutator in somatic hypermutation of immunoglobulin variable genes
journal contribution
posted on 2023-06-08, 08:44 authored by Xianmin Zeng, David B Winter, Cynthia Kasmer, Kenneth H Kraemer, Alan LehmannAlan Lehmann, Patricia J GearhartTo determine whether DNA polymerase plays a role in the hypermutation of immunoglobulin variable genes, we examined the frequency and pattern of substitutions in variable VH6 genes from the peripheral blood lymphocytes of three patients with xeroderma pigmentosum variant disease, whose polymerase had genetic defects. The frequency of mutation was normal but the types of base changes were different: there was a decrease in mutations at A and T and a concomitant rise in mutations at G and C. We propose that more than one polymerase contributes to hypermutation and that if one is absent, others compensate. The data indicate that polymerase is involved in generating errors that occur predominantly at A and T and that another polymerase(s) may preferentially generate errors opposite G and C.
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Publication status
- Published
Journal
Nature ImmunologyISSN
1529-2908Publisher
Nature Publishing GroupExternal DOI
Volume
2Page range
537-541Pages
5.0Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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