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Download fileDaun02 inactivation of behaviorally-activated Fos-expressing neuronal ensembles
journal contribution
posted on 2023-06-09, 04:56 authored by Eisuke KoyaEisuke Koya, Gabriella Margretts-Smith, Bruce T HopeLearned associations about salient experiences (e.g. drug exposure, stress) and their associated environmental stimuli are mediated by a minority of sparsely distributed, behaviorally activated neurons coined ‘neuronal ensembles’. For many years, it was not known whether these neuronal ensembles played causal roles in mediating learned behaviors. However, in the last several years the ‘Daun02 inactivation technique’ in Fos-lacZ transgenic rats has proved very useful in establishing causal links between neuronal ensembles that express the activity-regulated protein ‘Fos’ and learned behaviors. Fosexpressing neurons in these rats also express the bacterial protein b-galactosidase (b-gal) in strongly activated neurons. When the prodrug Daun02 is injected into the brains of these rats 90 min after a behavior (e.g. drug-seeking) or cue exposure, then the Daun02 is converted into daunorubicin by b-gal, which selectively inactivates the Fos and b-galexpressing neurons that were activated 90 min before the Daun02 injection. This unit presents protocols for breeding the Fos-lacZ rats and conducting appropriate Daun02 inactivation experiments.
History
Publication status
- Published
File Version
- Accepted version
Journal
Current Protocols in NeuroscienceISSN
1934-8584Publisher
John Wiley & SonsExternal DOI
Issue
8Volume
76Page range
36.1-36.17Book title
Current protocols in neuroscienceDepartment affiliated with
- Psychology Publications
Full text available
- Yes
Peer reviewed?
- Yes