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Definition of an inflammatory biomarker signature in plasma-derived extracellular vesicles of glioblastoma patients

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posted on 2023-06-10, 02:23 authored by Chiara Cilibrasi, Thomas Simon, Marian Vintu, Christos Tolias, Mark SamuelsMark Samuels, Nektarios K Mazarakis, Murat Eravci, Nicolas Stewart, Giles Critchley, Georgios GiamasGeorgios Giamas
Glioblastoma (GB) is an aggressive type of tumour for which therapeutic options and biomarkers are limited. GB diagnosis mostly relies on symptomatic presentation of the tumour and, in turn, brain imaging and invasive biopsy that can delay its diagnosis. Description of easily accessible and effective biomarkers present in biofluids would thus prove invaluable in GB diagnosis. Extracellular vesicles (EVs) derived from both GB and stromal cells are essential to intercellular crosstalk in the tumour bulk, and circulating EVs have been described as a potential reservoir of GB biomarkers. Therefore, EV-based liquid biopsies have been suggested as a promising tool for GB diagnosis and follow up. To identify GB specific proteins, sEVs were isolated from plasma samples of GB patients as well as healthy volunteers using differential ultracentrifugation, and their content was characterised through mass spectrometry. Our data indicate the presence of an inflammatory biomarker signature comprising members of the complement and regulators of inflammation and coagulation including VWF, FCGBP, C3, PROS1, and SERPINA1. Overall, this study is a step forward in the development of a non-invasive liquid biopsy approach for the identification of valuable biomarkers that could significantly improve GB diagnosis and, consequently, patients’ prognosis and quality of life.

History

Publication status

  • Published

File Version

  • Published version

Journal

Biomedicines

ISSN

2227-9059

Publisher

MDPI

Issue

1

Volume

10

Page range

1-15

Article number

a125

Department affiliated with

  • Biochemistry Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2022-01-20

First Open Access (FOA) Date

2022-01-20

First Compliant Deposit (FCD) Date

2022-01-20

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