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Determination of protein charge by capillary zone electrophoresis
journal contribution
posted on 2023-06-07, 19:56 authored by Donald J Winzor, Susan Jones, Stephen E HardingThe feasibility of employing classical electrophoresis theory to determine the net charge (valence) of proteins by capillary zone electrophoresis is illustrated in this paper. An outline of a procedure to facilitate the interpretation of mobility measurements is demonstrated by its application to a published mobility measurement for Staphylococcal nuclease at pH 8.9 that had been obtained by capillary zone electrophoresis. The significantly higher valence of +7.5 (cf. 5.6 from the same series of measurements) that has been reported on the basis of a "charge ladder" approach for charge determination signifies the likelihood that the latter generic approach may be prone to error arising from nonconformity of the experimental system with an inherent assumption that chemical modification or mutation of amino acid residues has no effect on the overall three-dimensional size and shape of the protein
History
Publication status
- Published
Journal
Analytical BiochemistryISSN
00032697External DOI
Issue
2Volume
333Page range
225-229Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes