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Development and validation of a novel rp-hplc method for pharmacokinetic studies of gliclazide in rat
journal contributionposted on 2023-06-08, 19:20 authored by R Talar, J Arshosaz, A Mostafavi, Ali Nokhodchi
A sensitive, simple and accurate reverse phase HPLC method was developed to determine plasma levels of gliclazide (GL) in rat. The method uses liquid-liquid extraction that is not expensive and ibuprofen as internal standard, that is easily available. Mean recovery for GL and internal standard was 80% and 82% respectively. Relative standard errors and CV% ranged from 0.47 to 8.99 and from 0.72 to 12.54% for intraday and interday HPLC injections, respectively. The limit of quantitation (LOQ) and the limit of detection (LOD) for GL in serum were 0.12 µg/mL and 0.06 µg/mL respectively. The developed HPLC method is a suitable and rapid method to be adopted for pharmacokinetic studies in rat and human.
PublisherRomanian Society for Pharmaceutical Sciences
Department affiliated with
- Chemistry Publications
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