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Direct object resolution by image subtraction: a new molecular ruler for nanometric measurements on complexed fluorophores
journal contribution
posted on 2023-06-15, 14:02 authored by Mark Osborne, Remi BoulineauA technique for measuring distances between two or more fluorophores spaced in the 10-100 nm range is described. We identify a linear correlation between the intensity-amplitude in the difference-image of single molecules undergoing fluorescence fluctuations and their separation. The transform is used to map distances between coupled fluorophores.
History
Publication status
- Published
File Version
- Accepted version
Journal
Chemical CommunicationsISSN
1359-7345Publisher
Royal Society of ChemistryExternal DOI
Issue
1Volume
49Page range
5559-5561Department affiliated with
- Chemistry Publications
Full text available
- No
Peer reviewed?
- Yes