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Dynamic clamp with StdpC software

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posted on 2023-06-07, 20:34 authored by Ildiko KemenesIldiko Kemenes, Vincenzo Marra, Michael CrossleyMichael Crossley, Dávid Samu, Kevin StarasKevin Staras, George KemenesGeorge Kemenes, Thomas NowotnyThomas Nowotny
Dynamic clamp is a powerful method that allows the introduction of artificial electrical components into target cells to simulate ionic conductances and synaptic inputs. This method is based on a fast cycle of measuring the membrane potential of a cell, calculating the current of a desired simulated component using an appropriate model and injecting this current into the cell. Here we present a dynamic clamp protocol using free, fully integrated, open-source software (StdpC, for spike timing-dependent plasticity clamp). Use of this protocol does not require specialist hardware, costly commercial software, experience in real-time operating systems or a strong programming background. The software enables the configuration and operation of a wide range of complex and fully automated dynamic clamp experiments through an intuitive and powerful interface with a minimal initial lead time of a few hours. After initial configuration, experimental results can be generated within minutes of establishing cell recording.

Funding

RCUK Academic Fellowship; Thomas Nowotny; RCUK

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Nature Protocols

ISSN

1754-2189

Publisher

Nature Publishing Group

Issue

3

Volume

6

Page range

405-417

Department affiliated with

  • Informatics Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

First Open Access (FOA) Date

2016-03-22

First Compliant Deposit (FCD) Date

2014-03-04

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