fmicb-09-03041.pdf (1.58 MB)
Elimination of ribosome inactivating factors improves the efficiency of and cell-free translation systems
journal contribution
posted on 2023-06-09, 18:24 authored by Tetiana Brodiazhenko, Marcus J O Johansson, Hiraku Takada, Tracy Nissan, Vasili Hauryliuk, Victoriia MurinaCell-free translation systems based on cellular lysates optimized for protein synthesis have multiple applications both in basic and applied science, ranging from studies of translational regulation to cell-free production of proteins and ribosome-nascent chain complexes. In order to achieve both high activity and reproducibility in a translation system, it is essential that the ribosomes in the cellular lysate are enzymatically active. Here we demonstrate that genomic disruption of genes encoding ribosome inactivating factors - HPF in and Stm1 in - robustly improve the activities of bacterial and yeast translation systems. Importantly, the elimination of HPF results in a complete loss of 100S ribosomes, which otherwise interfere with disome-based approaches for preparation of stalled ribosomal complexes for cryo-electron microscopy studies.
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Publication status
- Published
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- Published version
Journal
Frontiers in MicrobiologyISSN
1664-302XPublisher
Frontiers MediaExternal DOI
Issue
3041Volume
9Page range
1-9Department affiliated with
- Biochemistry Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2019-07-15First Open Access (FOA) Date
2019-07-15First Compliant Deposit (FCD) Date
2019-07-15Usage metrics
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