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Expression, purification and preliminary X-ray analysis of the BRCT domain from Rhp9/Crb2
journal contributionposted on 2023-06-07, 13:55 authored by J Hinks, Mark Roe, J C Ho, Felicity Watts, J Phelan, M McAllister, Laurence PearlLaurence Pearl
The BRCT domain from Rhp9 (a Schizosaccharomyces pombe DNA-damage checkpoint protein) has been expressed, purified and crystallized. Overexpression in bacterial cells was achieved by minimizing aeration during host cell growth. A robotic screen was used to determine the solubility parameters; concentration of the protein was achieved by exploiting this information. Single crystals suitable for X-ray analysis were obtained in two forms by vapour diffusion (trigonal, unit-cell parameters a = b = 228.04, c = 70.42 A, and tetragonal, P4/m Laue group symmetry, unit-cell parameters a = b = 72.3, c = 91.1 A).
JournalActa Crystallographica Section D: Biological Crystallography
Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
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