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Expression, purification and preliminary X-ray analysis of the BRCT domain from Rhp9/Crb2

journal contribution
posted on 2023-06-07, 13:55 authored by J Hinks, Mark Roe, J C Ho, Felicity Watts, J Phelan, M McAllister, Laurence PearlLaurence Pearl
The BRCT domain from Rhp9 (a Schizosaccharomyces pombe DNA-damage checkpoint protein) has been expressed, purified and crystallized. Overexpression in bacterial cells was achieved by minimizing aeration during host cell growth. A robotic screen was used to determine the solubility parameters; concentration of the protein was achieved by exploiting this information. Single crystals suitable for X-ray analysis were obtained in two forms by vapour diffusion (trigonal, unit-cell parameters a = b = 228.04, c = 70.42 A, and tetragonal, P4/m Laue group symmetry, unit-cell parameters a = b = 72.3, c = 91.1 A).

History

Publication status

  • Published

Journal

Acta Crystallographica Section D: Biological Crystallography

ISSN

0907-4449

Issue

Pt 7

Volume

59

Page range

1230-1233.

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Notes

GDSC62

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2007-03-26

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