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Identification of a DNA nonhomologous end-joining complex in bacteria
journal contribution
posted on 2023-06-07, 13:33 authored by Geoffrey R Weller, Boris Kysela, Rajat Roy, Louise M Tonkin, Elizabeth Scanlan, Marina Della, Susanne Krogh Devine, Jonathan P Day, Adam Wilkinson, Fabrizio d'Adda di Fagagna, Kevin M Devine, Richard P Bowater, Penny Jeggo, Stephen P Jackson, Aidan DohertyAidan DohertyIn eukaryotic cells, double-strand breaks (DSBs) in DNA are generally repaired by the pathway of homologous recombination or by DNA nonhomologous end joining (NHEJ). Both pathways have been highly conserved throughout eukaryotic evolution, but no equivalent NHEJ system has been identified in prokaryotes. The NHEJ pathway requires a DNA end-binding component called Ku. We have identified bacterial Ku homologs and show that these proteins retain the biochemical characteristics of the eukaryotic Ku heterodimer. Furthermore, we show that bacterial Ku specifically recruits DNA ligase to DNA ends and stimulates DNA ligation. Loss of these proteins leads to hypersensitivity to ionizing radiation in Bacillus subtilis. These data provide evidence that many bacteria possess a DNA DSB repair apparatus that shares many features with the NHEJ system of eukarya and suggest that this DNA repair pathway arose before the prokaryotic and eukaryotic lineages diverged.
History
Publication status
- Published
Journal
ScienceISSN
1095-9203Publisher
American Association for the Advancement of ScienceExternal DOI
Issue
5587Volume
297Page range
1686-9Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2006-11-27Usage metrics
Categories
No categories selectedKeywords
AntigensNuclear Bacillus subtilis/ genetics Bacterial Proteins/metabolism Binding Sites DNA Damage DNA Helicases DNA Ligases/ metabolism DNA Repair DNABacterial/ biosynthesis/metabolism DNA-Binding Proteins/metabolism Mutation Nuclear Proteins/metabolism Protein Binding Research SupportNon-U.S. Gov't Saccharomyces cerevisiae Proteins