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Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex

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journal contribution
posted on 2023-06-07, 14:41 authored by Elaine M Taylor, Alice CopseyAlice Copsey, Jessica HudsonJessica Hudson, Susanne Vidot, Alan LehmannAlan Lehmann
The SMC protein complexes play important roles in chromosome dynamics. The function of the SMC5-6 complex remains unclear, though it is involved in resolution of different DNA structures by recombination. We have now identified and characterized the four non-SMC components of the human complex and in particular demonstrated that the MAGEG1 protein is part of this complex. MAGE proteins play important but as yet undefined roles in carcinogenesis, apoptosis, and brain development. We show that, with the exception of the SUMO ligase hMMS21/hNSE2, depletion of any of the components results in degradation of all the other components. Depletion also confers sensitivity to methyl methanesulfonate. Several of the components are modified by sumoylation and ubiquitination.

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Molecular and Cellular Biology

ISSN

0270-7306

Publisher

American Society for Microbiology

Issue

4

Volume

28

Page range

1197-1206

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2008-07-17

First Open Access (FOA) Date

2018-05-11

First Compliant Deposit (FCD) Date

2018-05-11