The FASEB Journal - 2022 - Checconi - Influenza virus replication is affected by glutaredoxin1%E2%80%90mediated protein.pdf (1.7 MB)
Influenza virus replication is affected by glutaredoxin1-mediated protein deglutathionylation
journal contribution
posted on 2023-06-15, 15:51 authored by Paola Checconi, Cristiana Coni, Dolores Limongi, Sara Baldelli, Fabio Ciccarone, Marta De Angelis, Manuela MengozziManuela Mengozzi, Pietro Ghezzi, Maria Rosa Ciriolo, Lucia Nencioni, Anna Teresa PalamaraSeveral redox modifications have been described during viral infection, including influenza virus infection, but little is known about glutathionylation and this respiratory virus. Glutathionylation is a reversible, post-translational modification, in which protein cysteine forms transient disulfides with glutathione (GSH), catalyzed by cellular oxidoreductases and in particular by glutaredoxin (Grx). We show here that (i) influenza virus infection induces protein glutathionylation, including that of viral proteins such as hemagglutinin (HA); (ii) Grx1-mediated deglutathionylation is important for the viral life cycle, as its inhibition, either with an inhibitor of its enzymatic activity or by siRNA, decreases viral replication. Overall these data contribute to the characterization of the complex picture of redox regulation of the influenza virus replication cycle and could help to identify new targets to control respiratory viral infection.
History
Publication status
- Published
File Version
- Published version
Journal
The FASEB JournalISSN
0892-6638Publisher
WileyExternal DOI
Issue
2Volume
37Page range
1-12Department affiliated with
- Clinical and Experimental Medicine Publications
Full text available
- Yes
Peer reviewed?
- Yes