Interaction sites of the Epstein-Barr virus Zta transcription factor with the host genome in epithelial cells.pdf (717.74 kB)
Interaction sites of the Epstein-Barr virus Zta transcription factor with the host genome in epithelial cells
journal contribution
posted on 2023-06-10, 02:43 authored by Anja Godfrey, Kay OsbornKay Osborn, Alison SinclairEpstein-Barr virus (EBV) is present in a state of latency in infected memory B-cells and EBV-associated lymphoid and epithelial cancers. Cell stimulation or differentiation of infected B-cells and epithelial cells induces reactivation to the lytic replication cycle. In each cell type, the EBV transcription and replication factor Zta (BZLF1, EB1) plays a role in mediating the lytic cycle of EBV. Zta is a transcription factor that interacts directly with Zta response elements (ZREs) within viral and cellular genomes. Here we undertake chromatin-precipitation coupled to DNA-sequencing (ChIP-Seq) of Zta-associated DNA from cancer-derived epithelial cells. The analysis identified over 14?000 Zta-binding sites in the cellular genome. We assessed the impact of lytic cycle reactivation on changes in gene expression for a panel of Zta-associated cellular genes. Finally, we compared the Zta-binding sites identified in this study with those previously identified in B-cells and reveal substantial conservation in genes associated with Zta-binding sites.
History
Publication status
- Published
File Version
- Published version
Journal
Access MicrobiolISSN
2516-8290Publisher
Microbiology SocietyExternal DOI
Issue
11Volume
3Page range
1-7Event location
EnglandDepartment affiliated with
- Biochemistry Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2022-02-24First Open Access (FOA) Date
2022-02-24First Compliant Deposit (FCD) Date
2022-02-24Usage metrics
Categories
No categories selectedLicence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC