File(s) not publicly available
Localization of Y-Family Polymerases and the DNA Polymerase Switch in Mammalian Cells
journal contribution
posted on 2023-06-07, 22:58 authored by Patricia Kannouche, Alan LehmannAlan LehmannDuring translesion synthesis past sites of damaged DNA, specialized Y-family polymerases are employed by the cell to replace the high stringency replicative polymerases and synthesize DNA past the damaged site. These polymerases are localized in replication factories during the S phase of the cell cycle. When progress of the replication fork is blocked, the polymerase accessory protein, proliferating cell nuclear antigen (PCNA), becomes ubiquitinated and the monoubiquitinated PCNA has an increased affinity for Y-family DNA polymerase ¿ (pol¿). This chapter describes methods for visualizing the polymerases in replication factories, for analyzing the ubiquitination status of PCNA, and for measuring its interaction with pol¿ in chromatin extracts.
History
Publication status
- Published
Journal
Methods in EnzymologyISSN
0076-6879Publisher
ElsevierExternal DOI
Volume
408Page range
407-415Pages
9.0Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- No
Peer reviewed?
- Yes