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Measurement of chromosomal DNA single-strand breaks and replication fork progression rates
journal contributionposted on 2023-06-07, 22:10 authored by Claire Breslin, Paula M Clements, Sherif F El-Khamisy, Eva Petermann, Natasha Iles, Keith CaldecottKeith Caldecott
Chromosomal single-strand breaks (SSBs) are the most common lesions arising in cells, but are normally rapidly repaired by multiprotein complexes centered around the scaffold protein, XRCC1. Here, we describe protocols to measure chromosomal SSBs in cells and for recovering and identifying novel components of SSBR complexes in vitro and in vivo. We also describe an assay we employ to measure the rate of replication fork progression in mammalian/vertebrate cells in the presence or absence of DNA damage.
JournalMethods in Enzymology
Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
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