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Mutation of a Single Amino Acide Residue in the Basic Region of the Epstein-Barr Virus (EBV) Lytic Cycle Switch Protein Zta (BZLF1) Prevents Reactivation of EBV from Latency
journal contributionposted on 2023-06-07, 21:52 authored by Celine Schelcher, Sarah Valencia, Henri-Jacques Delecluse, Matthew Hicks, Alison Sinclair
Zta, the product of the BZLF1 gene carried by Epstein-Barr virus (EBV), is crucial for reactivation of EBV from latency. Zta is a member of the bZIP family of transcription factors, and in common with many of these, Zta possesses a conserved cysteine residue in its basic region (C189) and a further cysteine residue in its ZIP region (C222). We demonstrate that C189 is required to reactivate EBV from latency but C222 is not and that this single amino acid affects two independent functions of Zta, (i) binding to a Zta-responsive site and (ii) manipulating the cell cycle.
JournalJournal of Virology
PublisherAmerican Society for Microbiology
Department affiliated with
- Biochemistry Publications
NotesThis research was undertaken in Dr Sinclair's group at the University of Sussex, in collaboration with Prof Delecluse. The work identifies the importance of a residue in the DNA contact region of a viral transcription factor for viral replication.
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