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Download fileNew technologies for examining neuronal ensembles in drug addiction and fear
journal contribution
posted on 2023-06-09, 04:56 authored by Fabio C Cruz, Eisuke KoyaEisuke Koya, Danielle H Guez-Barber, Jennifer M Bossert, Carl R Lupica, Yavin Shaham, Bruce T HopeCorrelational data suggest that learned associations are encoded within neuronal ensembles. However, it has been difficult to prove that neuronal ensembles mediate learned behaviours because traditional pharmacological and lesion methods, and even newer cell type-specific methods, affect both activated and non-activated neurons. Additionally, previous studies on synaptic and molecular alterations induced by learning did not distinguish between behaviourally activated and non-activated neurons. Here, we describe three new approaches—Daun02 inactivation, FACS sorting of activated neurons and c-fos-GFP transgenic rats — that have been used to selectively target and study activated neuronal ensembles in models of conditioned drug effects and relapse. We also describe two new tools — c-fos-tTA mice and inactivation of CREB-overexpressing neurons — that have been used to study the role of neuronal ensembles in conditioned fear.
History
Publication status
- Published
File Version
- Accepted version
Journal
Nature Reviews NeuroscienceISSN
1471-003XPublisher
Nature ResearchExternal DOI
Volume
14Page range
743-754Department affiliated with
- Psychology Publications
Full text available
- Yes
Peer reviewed?
- Yes