Anotida Madzvamuse 12-Published-4.12.20.pdf (7.96 MB)
Optogenetic tuning reveals rho amplification-dependent dynamics of a cell contraction signal network
journal contribution
posted on 2023-06-09, 22:22 authored by Dominic Kamps, Johannes Koch, Victor O Juma, Eduard Campillo-Funollet, Melanie Graessl, Soumya Banerjee, Tomáš Mazel, Xi Chen, Yao-Wen Wu, Stephanie Portet, Anotida MadzvamuseAnotida Madzvamuse, Perihan Nalbant, Leif DehmeltLocal cell contraction pulses play important roles in tissue and cell morphogenesis. Here, we improve a chemo-optogenetic approach and apply it to investigate the signal network that generates these pulses. We use these measurements to derive and parameterize a system of ordinary differential equations describing temporal signal network dynamics. Bifurcation analysis and numerical simulations predict a strong dependence of oscillatory system dynamics on the concentration of GEF-H1, an Lbc-type RhoGEF, which mediates the positive feedback amplification of Rho activity. This prediction is confirmed experimentally via optogenetic tuning of the effective GEF-H1 concentration in individual living cells. Numerical simulations show that pulse amplitude is most sensitive to external inputs into the myosin component at low GEF-H1 concentrations and that the spatial pulse width is dependent on GEF-H1 diffusion. Our study offers a theoretical framework to explain the emergence of local cell contraction pulses and their modulation by biochemical and mechanical signals.
History
Publication status
- Published
File Version
- Published version
Journal
Cell RepISSN
2211-1247Publisher
Cell PressExternal DOI
Issue
9Volume
33Page range
1-14Article number
a108467Event location
United StatesDepartment affiliated with
- Mathematics Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2020-12-08First Open Access (FOA) Date
2020-12-08First Compliant Deposit (FCD) Date
2020-12-07Usage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC