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Paxillin associates with Poly(A)-binding protein 1 at the dense endoplasmic reticulum and the leading edge of migrating cells
journal contribution
posted on 2023-06-08, 00:50 authored by Alison J Woods, Marnie S Roberts, Jyoti Choudhary, Simon T Barry, Yuichi Mazaki, Hisataka Sabe, Simon Morley, David R Critchley, Jim C NormanUsing mass spectrometry we have identified proteins which co-immunoprecipitate with paxillin, an adaptor protein implicated in the integrin-mediated signaling pathways of cell motility. A major component of paxillin immunoprecipitates was poly(A)-binding protein 1, a 70-kDa mRNA-binding protein. Poly(A)-binding protein 1 associated with both the a and ß isoforms of paxillin, and this was unaffected by RNase treatment consistent with a protein-protein interaction. The NH2-terminal region of paxillin (residues 54¿313) associated directly with poly(A)-binding protein 1 in cell lysates, and with His-poly(A)-binding protein 1 immobilized in microtiter wells. Binding was specific, saturable and of high affinity (K d of ¿10 nm). Cell fractionation studies showed that at steady state, the bulk of paxillin and poly(A)-binding protein 1 was present in the ¿dense¿ polyribosome-associated endoplasmic reticulum. However, inhibition of nuclear export with leptomycin B caused paxillin and poly(A)-binding protein 1 to accumulate in the nucleus, indicating that they shuttle between the nuclear and cytoplasmic compartments. When cells migrate, poly(A)-binding protein 1 colocalized with paxillin-ß at the tips of lamellipodia. Our results suggest a new mechanism whereby a paxillipoly(A)-binding protein 1 complex facilitates transport of mRNA from the nucleus to sites of protein synthesis at the endoplasmic reticulum and the leading lamella during cell migration.
History
Publication status
- Published
Journal
Journal of Biological ChemistryISSN
0021-9258Publisher
American Society for Biochemistry and Molecular BiologyExternal DOI
Issue
8Volume
277Page range
6428-6437Pages
10.0Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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