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eji.202049101 Peptide microarray based.pdf (2.13 MB)
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Peptide microarray based analysis of antibody responses to SARS-CoV-2 identifies unique epitopes with potential for diagnostic test development

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posted on 2023-06-09, 23:29 authored by Pavlo Holenya, Paul Joris Lange, Ulf Reimer, Wolfram Woltersdorf, Thomas Panterodt, Michael Glas, Mark Wasner, Maren Eckey, Michael Drosch, Jörg-Michael Hollidt, Michael Naumann, Florian KernFlorian Kern, Holger Wenschuh, Robert Lange, Karsten Schnatbaum, Frank F Bier
Humoral immunity to the Severe Adult Respiratory Syndrome (SARS) Coronavirus (CoV)-2 is not fully understood yet but is a crucial factor of immune protection. The possibility of antibody cross-reactivity between SARS-CoV-2 and other human coronaviruses (HCoVs) would have important implications for immune protection but also for the development of specific diagnostic ELISA tests. Using peptide microarrays, n = 24 patient samples and n = 12 control samples were screened for antibodies against the entire SARS-CoV-2 proteome as well as the Spike (S), Nucleocapsid (N), VME1 (V), R1ab, and Protein 3a (AP3A) of the HCoV strains SARS, MERS, OC43 and 229E. While widespread cross-reactivity was revealed across several immunodominant regions of S and N, IgG binding to several SARS-CoV-2-derived peptides provided statistically significant discrimination between COVID-19 patients and controls. Selected target peptides may serve as capture antigens for future, highly COVID-19-specific diagnostic antibody tests.


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European Journal of Immunology





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