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Phage display in the study of infectious diseases
journal contribution
posted on 2023-06-08, 14:09 authored by Lisa MullenLisa Mullen, Sean P Nair, John M Ward, Andrew N Rycroft, Brian HendersonMicrobial infections are dependent on the panoply of interactions between pathogen and host and identifying the molecular basis of such interactions is necessary to understand and control infection. Phage display is a simple functional genomic methodology for screening and identifying protein-ligand interactions and is widely used in epitope mapping, antibody engineering and screening for receptor agonists or antagonists. Phage display is also used widely in various forms, including the use of fragment libraries of whole microbial genomes, to identify peptide-ligand and protein-ligand interactions that are of importance in infection. In particular, this technique has proved successful in identifying microbial adhesins that are vital for colonization.
History
Publication status
- Published
Journal
Trends in MicrobiologyISSN
0966-842XPublisher
ElsevierExternal DOI
Issue
3Volume
14Page range
141-147Department affiliated with
- Clinical and Experimental Medicine Publications
Full text available
- No
Peer reviewed?
- Yes