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Photo-CIDNP NMR spectroscopy of a heme-containing protein
journal contributionposted on 2023-06-07, 21:01 authored by Iain Day, Rachel Wain, Kaeko Tozawa, Lorna J. Smith, P. J. Hore
There are relatively few examples of the application of photo-CIDNP NMR spectroscopy to chromophore-containing proteins. The most likely reason for this is that simultaneous absorption of light by the photosensitiser molecule and the protein chromophore reduces the effectiveness of the photochemical reaction that produces the observed nuclear polarisation. We present details of exper- iments performed on the air-oxidised form of a small cytochrome, from the thermophilic bacterium Hydrogenobacter thermophilus, using both the wild-type protein and apo and holo forms of a double alanine b-type mutant. We show that, along with the apo state, it is possible to generate CIDNP in the air-oxidised form of the b-type mutant, but not in the corresponding c-type cytochrome. This finding is supported by control experiments using horse-heart cytochrome c.
JournalJournal of Magnetic Resonance
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