Profiling non coding RNA expression in cerebrospinal fluid of amyotrophic lateral sclerosis patients.pdf (2.79 MB)
Profiling non-coding RNA expression in cerebrospinal fluid of amyotrophic lateral sclerosis patients
journal contribution
posted on 2023-06-10, 05:17 authored by Greig JoilinGreig Joilin, Elizabeth Gray, Alexander G Thompson, Kevin Talbot, Nigel LeighNigel Leigh, Sarah NewburySarah Newbury, Martin R Turner, Majid HafezparastMajid HafezparastIntroduction Objective biomarkers for the fatal neurodegenerative disease amyotrophic lateral sclerosis or motor neuron disease (ALS/MND) are critical for diagnosis, drug development, clinical trials, and insight into disease pathology. Key candidates for biomarkers present in biofluids include non-coding RNA (ncRNA) transcripts including microRNA, piwi-interacting RNA and transfer RNA. To determine if the central nervous system was the source of the dysregulated ncRNA biomarkers we previously observed in serum, we sought to identify dysregulated ncRNA candidates in cerebrospinal fluid (CSF) which may provide new insight into the disease pathology. Methods and materials Small RNA sequencing (RNA-seq) was undertaken on CSF samples from healthy controls (n?=?18), disease mimics (n?=?8), and ALS patients (n?=?40) in our Oxford Study for Biomarkers of ALS cohort, with RT-qPCR used to confirm their dysregulation. Results We found a range of ncRNA that were dysregulated in the RNA-seq screen, but these failed to be validated or detected in some cases using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Additionally, our previously identified serum ncRNA biomarker showed no change in CSF or correlation to serum. Conclusions This study suggests the CSF may not be the source of dysregulated ncRNA in the serum and highlights the difficulty in identifying ncRNA in CSF as biomarkers for ALS.
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Publication status
- Published
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- Published version
Journal
Annals of MedicineISSN
0785-3890Publisher
Informa UK LimitedExternal DOI
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1Volume
54Page range
3069-3078Department affiliated with
- Clinical and Experimental Medicine Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2022-11-01First Open Access (FOA) Date
2022-11-01First Compliant Deposit (FCD) Date
2022-10-31Usage metrics
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