1-s2.0-S2211124714001193-main.pdf (2.32 MB)
Protein phosphatase 1 recruitment by Rif1 regulates DNA replication origin firing by counteracting DDK activity
journal contribution
posted on 2023-06-08, 17:02 authored by Anoushka DaveAnoushka Dave, Carol Cooley, Mansi Garg, Alessandro BianchiAlessandro BianchiThe eukaryotic genome is replicated according to a strict temporal program. Here, Bianchi and colleagues find that Rif1, a master regulator of the DNA replication program in yeast and mammals, exerts its effect on DNA replication origins by recruiting protein phosphatase 1 (PP1) to chromosomes. Rif1/PP1 counteracts the positive action of the DDK kinase on the replicative kinase MCM. In a final twist, kinase action on Rif1 is proposed to eventually release PP1 and allow origin firing.
Funding
Analysis of regulation telomerase action & telomore capping function by the telomeric complex (Fellow: Bianchi); G0129; MRC-MEDICAL RESEARCH COUNCIL; G0701428
History
Publication status
- Published
File Version
- Published version
Journal
Cell ReportsISSN
2211-1247Publisher
ElsevierExternal DOI
Issue
1Volume
7Page range
53-61Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- Yes
Peer reviewed?
- Yes