Version 2 2023-06-12, 08:55Version 2 2023-06-12, 08:55
Version 1 2023-06-09, 16:15Version 1 2023-06-09, 16:15
journal contribution
posted on 2023-06-12, 08:55authored byGeorg Kustatscher, Nadia Hégarat, Karen L H Willis, Cristina Furlan, Jimi-Carlo Bukowski-Wills, Helfrid HocheggerHelfrid Hochegger, Juri Rappsilber
Chromatin proteins mediate replication, regulate expression and ensure integrity of the genome. So far, a comprehensive inventory of interphase chromatin has not been determined. This is largely due to its heterogeneous and dynamic composition, which makes conclusive biochemical purification difficult, if not impossible. As a fuzzy organelle it defies classical organellar proteomics and cannot be described by a single and ultimate list of protein components. Instead we propose a new approach that provides a quantitative assessment of a protein’s probability to function in chromatin. We integrate chromatin composition over a range of different biochemical and biological conditions. This resulted in interphase chromatin probabilities for 7635 human proteins, including 1840 previously uncharacterized proteins. We demonstrate the power of our large-scale data-driven annotation during the analysis of CDK regulation in chromatin. Quantitative protein ontologies may provide a general alternative to list-based investigations of organelles and complement Gene Ontology.
Funding
Exploiting chemical genetics to investigate the control of microtubule dynamics by mitotic kinases; G0900; CANCER RESEARCH UK; C28206/A14499