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Quantitative ion localization within Suaeda maritima leaf mesophyll cells
journal contributionposted on 2023-06-08, 11:30 authored by Diana M R Harvey, J L Hall, Tim Flowers, B Kent
Grown under saline conditions, Suaeda maritima accumulates Na+ and Cl- into its leaves, where individual mesophyll cells behave differently in their compartmentation of these ions. Measurements of ion concentrations within selected subcellular compartments show that freeze-substitution with dry sectioning is a valuable preparative technique for analytical electron microscopy of highly vacuolate plant material. Using this approach, absolute estimates were made of Na+, K+ and Cl- concentrations in the cytoplasm, cell walls, chloroplasts and vacuoles of leaf mesophyll cells.
Department affiliated with
- Evolution, Behaviour and Environment Publications
NotesTimes Cited: 113 Harvey, dmr hall, jl flowers, tj kent, b
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