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RsrA an anti-sigma factor regulated by redox change
journal contribution
posted on 2023-06-07, 21:04 authored by Ju-Gyeong Kang, Mark PagetMark Paget, Yeong-Jae Seok, Mi-Young Hahn, Jae-Bum Bae, Ji-Sook Hahn, Colin Kleanthous, Mark J Buttner, Jung-Hye RoeSigR (s(R)) is a sigma factor responsible for inducing the thioredoxin system in response to oxidative stress in the antibiotic-producing, Gram-positive bacterium Streptomyces coelicolor A3(2). Here we identify a redox-sensitive, s(R)-specific anti-sigma factor, RsrA, which binds s(R) and inhibits s(R)-directed transcription in vitro only under reducing conditions. Exposure to H2O2 or to the thiol-specific oxidant diamide caused the dissociation of the s(R)-RsrA complex, thereby allowing s(R)-dependent transcription. This correlated with intramolecular disulfide bond formation in RsrA. Thioredoxin was able to reduce oxidized RsrA, suggesting that s(R), RsrA and the thioredoxin system comprise a novel feedback homeostasis loop that senses and responds to changes in the intracellular thiol-disulfide redox balance.
History
Publication status
- Published
Journal
EMBO JournalISSN
02614189Publisher
Nature Publishing GroupExternal DOI
Issue
5Volume
18Page range
4292-4298ISBN
0261-4189Department affiliated with
- Biochemistry Publications
Notes
This was the first description of a new family of anti-sigma factors and RsrA was the first-described anti-sigma factor controlled by redox change.Full text available
- No
Peer reviewed?
- Yes