Schalbetter_et_al_manuscript_and_figure_legends_assembled.pdf (6.18 MB)
SMC complexes differentially compact mitotic chromosomes according to genomic context
journal contribution
posted on 2023-06-09, 07:50 authored by Stephanie Andrea Schalbettertter, Anton Goloborodko, Geoffrey Fudenberg, Jon-Matthew Belton, Catrina Miles, Miao Yu, Job Dekker, Leonid Mirny, Jon BaxterJon BaxterStructural maintenance of chromosomes (SMC) protein complexes are key determinants of chromosome conformation. Using Hi-C and polymer modelling, we study how cohesin and condensin, two deeply conserved SMC complexes, organize chromosomes in the budding yeast Saccharomyces cerevisiae. The canonical role of cohesin is to co-align sister chromatids, while condensin generally compacts mitotic chromosomes. We find strikingly different roles for the two complexes in budding yeast mitosis. First, cohesin is responsible for compacting mitotic chromosome arms, independently of sister chromatid cohesion. Polymer simulations demonstrate that this role can be fully accounted for through cis-looping of chromatin. Second, condensin is generally dispensable for compaction along chromosome arms. Instead, it plays a targeted role compacting the rDNA proximal regions and promoting resolution of peri-centromeric regions. Our results argue that the conserved mechanism of SMC complexes is to form chromatin loops and that distinct SMC-dependent looping activities are selectively deployed to appropriately compact chromosomes.
Funding
How does Condensin mediate topological change during mitosis?; G0865; Bbsrc-Biotechnology & Biological Sciences Research Council
History
Publication status
- Published
File Version
- Accepted version
Journal
Nature Cell BiologyISSN
1465-7392Publisher
Nature Publishing GroupExternal DOI
Issue
9Volume
19Page range
1071-1080Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Research groups affiliated with
- Genome Damage and Stability Centre Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2017-09-07First Open Access (FOA) Date
2018-02-21First Compliant Deposit (FCD) Date
2017-09-07Usage metrics
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