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Sigma-E is required for the production of the antibiotic actinomycin in Streptomyces antibioticus

journal contribution
posted on 2023-06-08, 05:08 authored by George H Jones, Mark PagetMark Paget, Leony Chamberlin, Mark J Buttner
The phsA gene encodes phenoxazinone synthase (PHS), which catalyses the penultimate step in the pathway for actinomycin biosynthesis in Streptomyces antibioticus. The phsA promoter strikingly resembles a putative Streptomyces s(E) cognate promoter, and purified Es(E) holoenzyme transcribed the phsA promoter in vitro. However, the phsA promoter was still active in an S. antibioticus sigE null mutant and the level of PHS activity was unaffected. Despite this, disruption of sigE blocked actinomycin production completely. The loss of actinomycin production correlated with a 10-fold decrease in the activity of actinomycin synthetase I, the enzyme which catalyses the activation of the precursor of the actinomycin chromophore.

History

Publication status

  • Published

Journal

Molecular Microbiology

ISSN

0950382X

Issue

1

Volume

23

Page range

169-178

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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