posted on 2024-04-29, 13:53authored byGeorge Cameron, Dominika T Gruszka, Rhian Gruar, Sherry Xie, Çağla Kaya, Kim A Nasmyth, Jon BaxterJon Baxter, Madhusudhan Srinivasan, Hasan Yardimci
Newly copied sister chromatids are tethered together by the cohesin complex, but how sister chromatid cohesion coordinates with DNA replication is poorly understood. Prevailing models suggest that cohesin complexes, bound to DNA before replication, remain behind the advancing replication fork to keep sister chromatids together. By visualizing single replication forks colliding with preloaded cohesin complexes, we find that the replisome instead pushes cohesin to where a converging replisome is met. Whereas the converging replisomes are removed during DNA replication termination, cohesin remains on nascent DNA and provides cohesion. Additionally, we show that CMG (CDC45-MCM2-7-GINS) helicase disassembly during replication termination is vital for proper cohesion in budding yeast. Together, our results support a model wherein sister chromatid cohesion is established during DNA replication termination.
Funding
Determining how EBV episome maintenance is regulated by TIMELESS function : MRC-MEDICAL RESEARCH COUNCIL | MR/X009432/1
History
Publication status
Published
File Version
Accepted version
Journal
Science
ISSN
0036-8075
Publisher
American Association for the Advancement of Science (AAAS)