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Site-specific human histone H3 methylation stability: fast K4me3 turnover
journal contribution
posted on 2023-06-08, 18:03 authored by Yupeng Zheng, Jeremiah D Tipton, Paul M Thomas, Neil L Kelleher, Steve M M SweetWe employ stable-isotope labeling and quantitative mass spectrometry to track histone methylation stability. We show that H3 trimethyl K9 and K27 are slow to be established on new histones and slow to disappear from old histones, with half-lives of multiple cell divisions. By contrast, the transcription-associated marks K4me3 and K36me3 turn over far more rapidly, with half-lives of 6.8 h and 57 h, respectively. Inhibition of demethylases increases K9 and K36 methylation, with K9 showing the largest and most robust increase. We interpret different turnover rates in light of genome-wide localization data and transcription-dependent nucleosome rearrangements proximal to the transcription start site.
History
Publication status
- Published
File Version
- Published version
Journal
ProteomicsISSN
1615-9853Publisher
WileyExternal DOI
Issue
19Volume
14Page range
2190-2199Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Notes
Keywords: Cell biology / Chromatin / Demethylation / Heterochromatin / Methylation turnover / SILAC / TranscriptionFull text available
- No
Peer reviewed?
- Yes