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Smc5/6 maintains stalled replication forks in a recombination-competent conformation

journal contribution
posted on 2023-06-07, 14:57 authored by Anja Irmisch, Eleni Ampatzidou, Ken'ichi Mizuno, Matthew J O'Connell, Jo Murray
The Smc5/6 structural maintenance of chromosomes complex is required for efficient homologous recombination (HR). Defects in Smc5/6 result in chromosome missegregation and fragmentation. By characterising two Schizosaccharomyces pombe smc6 mutants, we define two separate functions for Smc5/6 in HR. The first represents the previously described defect in processing recombination-dependent DNA intermediates when replication forks collapse, which leads to increased rDNA recombination. The second novel function defines Smc5/6 as a positive regulator of recombination in the rDNA and correlates mechanistically with a requirement to load RPA and Rad52 onto chromatin genome-wide when replication forks are stably stalled by nucleotide depletion. Rad52 is required for all HR repair, but Rad52 loading in response to replication fork stalling is unexpected and does not correlate with damage-induced foci. We propose that Smc5/6 is required to maintain stalled forks in a stable recombination-competent conformation primed for replication restart.

History

Publication status

  • Published

File Version

  • Submitted version

Journal

EMBO Journal

ISSN

0261-4189

Publisher

Nature Publishing Group

Issue

2

Volume

28

Page range

144-155

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Notes

On Endnotes Ref: GDSC271

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2009-02-23

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