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Sorting and pooling strategy: a novel tool to map a virus proteome for CD8 T-cell epitopes
journal contributionposted on 2023-06-08, 12:40 authored by Bernhard Ay, Mathias Streitz, Prisca Boisguerin, Andreas Schlosser, Carsten C. Mahrenholz, Sebastian D. Schuck, Florian KernFlorian Kern, Rudolf Volkmer
Human cytomegalovirus (CMV) is a major cause of morbidity in immunocompromised individuals. However, no efficient vaccine has been developed to date. Identification of T-cell target proteins and epitopes is crucial not only for developing a successful immunization strategy, but also for new approaches using adoptive transfer of antigen-specific T-cells. The CMV genome has more than 200 open reading frames potentially coding for as many proteins. Here, we describe a robust, fast, and simple SPOT synthesis strategy, which allowed us to micro-synthesize every possible CD8 T-cell epitope in the entire potential CMV proteome. So far, 9069 of these peptides have been tested in an ex vivo T-cell stimulation assay. As well as confirming a number of previously known epitopes, we identified several new ones.
ISSN0006-3525 (Print) 0006-3525 (Linking)
Department affiliated with
- Clinical and Experimental Medicine Publications
NotesAy, Bernhard Streitz, Mathias Boisguerin, Prisca Schlosser, Andreas Mahrenholz, Carsten C Schuck, Sebastian D Kern, Florian Volkmer, Rudolf Biopolymers. 2007;88(1):64-75.
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