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Specificity determinants for Cry insecticidal proteins: insights from their mode of action

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posted on 2023-06-09, 02:19 authored by Juan Luis Jurat-Fuentes, Neil CrickmoreNeil Crickmore
Insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are used as active components of biopesticides and as plant incorporated protectants in transgenic crops. One of the most relevant attributes of these Bt protein-based insecticidal technologies is their high specificity, which assures lack of detrimental effects on non-target insects, vertebrates and the environment. The identification of specificity determinants in Bt insecticidal proteins could guide risk assessment for novel insecticidal proteins currently considered for commercialization. In this work we review the available data on specificity determinants of crystal (Cry) insecticidal proteins as the Bt toxins most well characterized and used in transgenic crops. The multi step mode of action of the Cry insecticidal proteins allows various factors to potentially affect specificity determination and here we define seven levels that could influence specificity. The relative relevance of each of these determinants on efficacy of transgenic crops producing Cry insecticidal proteins is also discussed.

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Journal of Invertebrate Pathology

ISSN

0022-2011

Publisher

Elsevier

Volume

142

Page range

5-10

Department affiliated with

  • Biochemistry Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2016-08-01

First Open Access (FOA) Date

2017-07-30

First Compliant Deposit (FCD) Date

2016-08-01

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